Coding

Part:BBa_K357000:Design

Designed by: Anish Kapadia   Group: iGEM10_Toronto   (2010-06-23)

catechol 1 2-dioxygenase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was changed post-optimization from the original to remove a EcoR1 restriction site located around bp 514, to remove the restriction site while maintaining a conserved aa sequence.

Source

This part is designed from genomic sequence: 

AUTHORS   Nelson,K., Paulsen,I., Weinel,C., Dodson,R., Hilbert,H., Fouts,D.,

          Gill,S., Pop,M., Martins Dos Santos,V., Holmes,M., Brinkac,L.,

          Beanan,M., DeBoy,R., Daugherty,S., Kolonay,J., Madupu,R.,

          Nelson,W., White,O., Peterson,J., Khouri,H., Hance,I., Lee,P.,

          Holtzapple,E., Scanlan,D., Tran,K., Moazzez,A., Utterback,T.,

          Rizzo,M., Lee,K., Kosack,D., Moestl,D., Wedler,H., Lauber,J.,

          Hoheisel,J., Straetz,M., Heim,S., Kiewitz,C., Eisen,J., Timmis,K.,

          Duesterhoft,A., Tummler,B. and Fraser,C.

TITLE     Complete genome sequence and comparative analysis of the

          metabolically versatile Pseudomonas putida KT2440

JOURNAL   Environ. Microbiol. 4 (12), 799-808 (2002)


References